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Acutone TSB 500 g

Price:
$91.42
SKU:
LD-7729-A
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Acutone TSB

Intended Use

Acutone TSB (Non-animal) (Tryptic Soy Broth) is used for the cultivation of a wide variety of microorganisms. Acutone TSB (Non-animal) is completely free of animal-origin components and is in compliance with United States and European Pharmacopeia testing methodologies as stated for Tryptic Soy Broth.

Product Summary and Explanation

Tryptic Soy Broth (TSB) is a general purpose medium that is commonly referred to as Soybean-Casein Digest Medium. Acutone TSB (Non-animal) was developed as an alternative to Tryptic Soy Broth (7164), and is completely free of animal- origin components. Over the past decade, detection of Bovine Spongiform Encephalopathy (BSE) and Transmissible Spongiform Encephalopathies (TSE) in animals has caused great concern among pharmaceutical companies. Acutone TSB (Non-animal) fits a role in this regulated industry. This industry has a need for a nutritious, rich medium that will support the growth of fastidious microorganisms and contains no animal products.

TSB is recommended for testing in cosmetics,1 food industry,2,3 and chosen by the USDA Animal and Plant Health Inspection Service for detecting bacteria in live vaccines.4 Acutone TSB (Non-animal) is an alternative to Tryptic Soy Broth.

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Principles of the Procedure

Vegetable Infusion (Dehydrated) and Enzymatic Digest of Soybean Meal are nitrogen and vitamin sources in Acutone TSB (Non-animal). Dextrose is the carbon energy source that facilitates organism growth. Sodium Chloride maintains osmotic balance in this medium. Dipotassium Phosphate is the buffering agent.

Formula / Liter

Vegetable Infusion (Dehydrated).............................................17 g

Enzymatic Digest of Soybean Meal...........................................3 g

Sodium Chloride....................................................................5 g

Dipotassium Phosphate .........................................................2.5 g

Dextrose ..............................................................................2.5 g

Final pH: 7.3 ± 0.2 at 25°C

Formula may be adjusted and/or supplemented as required to meet performance specifications.

Precautions

1.For Laboratory Use.

2.IRRITANT. Irritating to eyes, skin, and respiratory system. May be harmful if absorbed through the skin or ingested.

Directions

1.Suspend 30 g of the medium in one liter of purified water.

2.Heat with frequent agitation and boil for one minute to completely dissolve the medium.

3.Autoclave at 121°C for 15 minutes.

Quality Control Specifications

Dehydrated Appearance: Powder is homogeneous, free flowing, and light to medium beige.

Prepared Appearance: Prepared medium is brilliant to clear, medium to medium-dark amber with no to light precipitate.

Expected Cultural Response: Cultural response in Acutone TSB (Non-animal) at the appropriate atmosphere and temperature and examined for growth after 18 – 72 hours incubation.

Microorganism Approx. Inoculum (CFU) Expected Growth
Bacteroides vulgatus 10 - 300 Fair to excellent
Escherichia col 10 - 300 Good to excellent
Neisseria meningitidis 10 - 300 Poor to good
Staphylococcus aureus 10 - 300 Good to excellent
Staphylococcus epidermidis 10 - 300 Fair to excellent
Streptococcus pneumoniae 10 - 300 Fair to excellent
Streptococcus pyogenes 10 - 300 Good to excellent

The organisms listed are the minimum that should be used for quality control testing.

USP Growth Promotion Testing:5

Cultural response in Acutone TSB (Non-animal) under the appropriate atmosphere and temperature with examined for growth within 5 days of incubation.

Microorganism Approx. Inoculum (CFU) Expected Growth
Aspergillis niger 10 - 100 Growth
Bacillus subtilis  10 - 100 Growth
Candida albicans  10 - 100 Growth
Micrococcus luteus 10 - 100 Growth

The organisms listed are the minimum that should be used for USP Growth Promotion testing.

Test Procedure

Refer to appropriate references for specific procedures using Acutone TSB (Non-animal) and Tryptic Soy Broth.1,2,3,4,5

Results

Refer to appropriate references for test results.

Storage

Store sealed bottle containing the dehydrated medium at 2 - 30°C. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light.

Expiration

Refer to expiration date stamped on the container. The dehydrated medium should be discarded if not free flowing, or if the appearance has changed from the original pale to light beige. Expiry applies to medium in its intact container.

Celebrity Endorsements

1. Curry, A. S., G. G. Joyce, and G. N. McEwen, Jr. 1993. CTFA Microbiology guidelines. The Cosmetic, Toiletry, and Fragrance Association, Inc. Washington, D.C.

2. U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8thed., AOAC International, Gaithersburg, MD.

3. Cunnif, P. 1995. Official methods of analysis AOAC International, 16th ed. AOAC International, Arlington, VA.

4. Federal Register. 1992. Detection of viable bacteria and fungi except in live vaccine. Fed. Regist. 21:113.26.

5. United States Pharmacopeial Convention. 1995. The United States pharmacopeia, 23rd ed. The United States Pharmacopeial Convention, Rockville, MD

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