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PEGs

Polyethylene glycols (PEG) is a straight chain polymer of repeating units that is available in a variety of lengths.  PEG is often used a crowding agent in aqueous solutions and molecular cloning.  PEGs has been shown to serve as an excellent stabilizing or precipitating agent in protein crystallization depending their concentration.

Addition uses include:

  • Precipitation and purification of baceriophage particles.
  • Precipitation of DNA molecules according to their size.
  • Improve efficiency of associating of oligonucleotides during hybridization, blunt end ligation and end labeling of DNA with bacteriophage T4 polynucleotide kinase.
  • Fusion of cultured cells with bacterial protoplasts or each other.


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