Cutter, A.D. (2006) Nucleotide polymorphism and linkage disequilibrium in wild populations of the partial selfer Caenorhabditis elegans. Genetics. Vol. 172, 171-184.
-The protocol for the Scottish nematode isolations in short is as follows: Small samples of compost (2 ml), or individual isopods (crushed, nine C. elegans strains from Porcellio scaber, P. spinicornis, and an unidentified species), were placed on standard 6-cm NGM-lite agar plates spotted with E. coli OP50. E. coli OP50 is a uracil auxotroph whose growth is limited on NGM plates. A limited bacterial lawn is desirable since it allows for easier observation and better mating of the worms. After 4 hours, individual nematodes were isolated on separate NGM-lite agar plates. Self-fertile individuals were inspected under 100x microscopy for morphological characters. Progeny of candidate Caenorhabditis were subjected to mating tests and species identity was confirmed in this work.
Yi, S.Y.; et. al. (2001) A single-stranded telomere binding protein in the nematode Caenorhabditis elegans. Federation of European Biochemical Societies (FEBS). Letters 505. 301-306.