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NGM Lite Agar 500 g

Price:
$75.00
SKU:
LE-3005-500G
Quantity:

NGM Lite Agar

Contents:

  Tryptone
  4 g/l
  NaCl
  2 g/l
  PO4
  25 mM
  Agar
  20 g/l

 

Preparation:

  1. Dissolve 29.5 g mix in 1 liter of water. 
  2. After autoclaving at 121 °C for 15 minutes.
  3. Add 1 ml of 5mg/ml Cholesterol to the medium.

Celebrity Endorsements

Cutter, A.D. (2006) Nucleotide polymorphism and linkage disequilibrium in wild populations of the partial selfer Caenorhabditis elegans. Genetics. Vol. 172, 171-184.

-The protocol for the Scottish nematode isolations in short is as follows: Small samples of compost (2 ml), or individual isopods (crushed, nine C. elegans strains from Porcellio scaber, P. spinicornis, and an unidentified species), were placed on standard 6-cm NGM-lite agar plates spotted with E. coli OP50. E. coli OP50 is a uracil auxotroph whose growth is limited on NGM plates. A limited bacterial lawn is desirable since it allows for easier observation and better mating of the worms. After 4 hours, individual nematodes were isolated on separate NGM-lite agar plates. Self-fertile individuals were inspected under 100x microscopy for morphological characters. Progeny of candidate Caenorhabditis were subjected to mating tests and species identity was confirmed in this work.

Yi, S.Y.; et. al. (2001) A single-stranded telomere binding protein in the nematode Caenorhabditis elegans. Federation of European Biochemical Societies (FEBS). Letters 505. 301-306.

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