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GDNF, human 2ug

Price:
$72.00
SKU:
TE-0006-2UG
Quantity:

GDNF, human

(Recombinant Human Glial Cell-derived Neurotrophic Factor)

Purity: >97% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Sterile Filtered White lyophilized powder

Amino Acid Sequence (135aa):

MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

The above sequence is of the monomer (~15.2 kDa), GDNF contains a disulphide between monomers creating a homodimeric (30.4 kDA) protein consisting of two 134 amino acid chains.

Reconstitution:

The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions.

Storage:

The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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