Loading... Please wait...
  • My Account
  • Order Status
  • Wish Lists

EE Broth Mossel Enrichment 500g

Price:
$120.00
SKU:
BD-256620-500G
Quantity:

EE Broth Mossel Enrichment

Intended Use

EE Broth Mossel Enrichment is used for selectively enriching and detecting Enterobacteriaceae, particularly from foods. Meets United States Pharmacopeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP)1-3 performance specifications, where applicable.

Summary and Explanation

EE Broth Mossel Enrichment is prepared according to the formula of Mossel, Visser and Cornelissen.4 The formula contains dextrose to facilitate growth of most Enterobacteriaceae, thus
ensuring the detection of Salmonella and other lactose-negative organisms. EE Broth Mossel Enrichment should be used as an enrichment broth, followed by a selective medium; e.g., Violet Red Bile Agar.
The enumeration of Enterobacteriaceae is of great concern in monitoring the sanitary condition of food. Enterobacteriaceae can be injured in food-processing procedures, which include 
exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives or sanitizers.5 Recovery relies on proper resuscitation of damaged cells. EE Broth Mossel Enrichment is used to detect and enumerate Enterobacteriaceae found per milliliter or per gram of test sample of food when performing the Most Probable Number (MPN) technique with pre-enrichment.6-7
EE Broth Mossel Enrichment is listed in the USP as one of the recommended media for the isolation of bile-tolerant gramnegative bacteria from nonsterile pharmaceutical products.1

Principles of the Procedure

Peptones provide nitrogen, vitamins and amino acids. Dextrose is a carbon source. Disodium phosphate and monopotassium phosphate are buffering agents. Brilliant green and oxgall are selective agents.

User Quality Control

NOTE: Differences in the Identity Specifications and Cultural Response testing for media offered as both Difco™ and BBL™ brands may reflect differences in the development and testing of media for industrial and clinical applications, per the referenced publications.
Identity Specifications
Difco™ EE Broth Mossel Enrichment
Dehydrated Appearance: Light green, free flowing, homogeneous.
Solution:                       4.5% solution, soluble in purified water. Solution
                                   is emerald green, clear.
Prepared Appearance:    Emerald green, clear.
Reaction of 4.5%
Solution at 25°C:           7.2 ± 0.2
Cultural Response
Difco™ EE Broth Mossel Enrichment
Prepare the medium per label directions. Inoculate 9 mL tubes and incubate at 35 ± 2°C for 18-24 hours and 48 hours, if necessary.

ORGANISM  ATCC™ INOCULUM CFU RECOVERY ACID
Enterobacter
aerogenes
13048  30-100 Good + (yellow)
Escherichia coli 25922  30-100 Good + (yellow)
Shigella boydii 12030 30-100 Good
Staphylococcus aureus 25923  30-100 Marked to
complete inhibition

Inoculate 100 mL bottles and incubate at 30-35°C for 18-24 hours and 48 hours, if necessary. Inoculate a 20 mL tube with Escherichia coli ATCC 8739 and incubate at 35-37°C for 18-48 hours.

ORGANISM ATCC™ INOCULUM
CFU
INCUBATION
TEMP
INCUBATION
TIME (HOURS)
RECOVERY
Escherichia coli 8739  <100 30-35° 24 Growth
Escherichia coli 8739  <100 35-37°C 18-48 Growth
Pseudomonas
aeruginosa
9027  <100 30-35°C 24 Growth
Staphylococcus
aureus 
6538  <100 30-35°C 48 No growth

Identity Specifications
BBL™ EE Broth Mossel Enrichment
Dehydrated Appearance: Fine, homogeneous and free of extraneous material.
Solution:                       4.5% solution, soluble in purified water. Solution
                                    is medium to dark green with or without a tint
                                    of yellow or blue; clear to slightly hazy.
Prepared Appearance:     Medium to dark green with or without a tint of
                                    yellow or blue; clear to slightly hazy.
Reaction of 4.5%
Solution at 25°C:            pH 7.2 ± 0.2
BBL™ EE Broth Mossel Enrichment (prepared)
Appearance:                 Medium to dark green and clear to trace hazy.
Reaction at 25°C:          pH 7.2 ± 0.2
Cultural Response
BBL™ EE Broth Mossel Enrichment
Prepare the medium per label directions. Inoculate 10 mL tubes and incubate at 35 ± 2°C for 18-24 hours and 48 hours, if necessary.

ORGANISM  ATCC™ INOCULUM CFU RECOVERY ACID
Escherichia coli 25922  103-104 Good + (yellow)
Pseudomonas
aeruginosa
10145  103-104 Good
Salmonella enterica
subsp. enterica
serotype Typhimurium
14028  103-104 Good + (yellow)
Shigella sonnei  9290  103-104 Good – to reduced
(yellow green)

Inoculate 100 mL bottles and incubate at 30-35°C for 18-24 hours and 48 hours, if necessary. Inoculate a 20 mL tube with Escherichia coli ATCC 8739 and incubate at 35-37°C for 18-48 hours.

ORGANISM ATCC™ INOCULUM
CFU
INCUBATION
TEMP
INCUBATION
TIME (HOURS)
RECOVERY
Escherichia coli 8739  <100 30-35° 24 Growth
Escherichia coli 8739  <100 35-37°C 18-48 Growth
Pseudomonas
aeruginosa
9027  <100 30-35°C 24 Growth
Staphylococcus
aureus 
6538  <100 30-35°C 48 No growth

BBL™ EE Broth Mossel Enrichment (prepared)
Inoculate 90 mL bottles and incubate as directed below.

ORGANISM ATCC™ INOCULUM
CFU
INCUBATION
TEMP
INCUBATION
TIME (HOURS) 
RECOVERY ACID
Enterobacter aerogenes 13048  102-103 35-37°C 18-48 Growth  + (yellow)
Escherichia coli 25922 102-103 35-37°C 18-48 Growth  + (yellow)
Salmonella enterica
subsp. enterica
serotype Typhimurium
13311  102-103 35-37°C 18-48 Growth  + (yellow)
Escherichia coli  8739  10-100 30-35° 18-24 Growth  + (yellow) to – or weak
Pseudomonas aeruginosa 9027  10-100 30-35° 18-24 Growth  N/A
Staphylococcus aureus 6538  102-103 30-35° 48 No growth

Formula

EE Broth Mossel Enrichment
Approximate Formula* Per Liter
Pancreatic Digest of Gelatin....................................... 10.0 g
Dextrose.................................................................. 5.0 g
Disodium Phosphate.................................................. 8.0 g
Monopotassium Phosphate......................................... 2.0 g
Brilliant Green......................................................... 15.0 mg
Oxgall................................................................... 20.0 g
EE Broth Mossel Enrichment
Approximate Formula* Per Liter
Pancreatic Digest of Gelatin....................................... 10.0 g
Dextrose.................................................................. 5.0 g
Oxgall.................................................................... 20.0 g
Disodium Phosphate.................................................. 8.0 g
Monopotassium Phosphate......................................... 2.0 g
Brilliant Green......................................................... 15.0 mg
*Adjusted and/or supplemented as required to meet performance criteria.

Directions for Preparation from Dehydrated Product

1. Suspend 45 g of the powder in 1 L of purified water.
2. Heat with frequent agitation until dissolved. DO NOT OVERHEAT. Media is heat sensitive.
3. Dispense into tubes or bottles as required.
4. Heat at 100°C in water bath or flowing steam for 30 minutes.
DO NOT AUTOCLAVE.
5. Test samples of the finished product for performance using stable, typical control cultures. Sample Collection and Handling For food samples, follow appropriate standard methods for details on sample collection and preparation according to sample type and geographic location.6-7
For pharmaceutical samples, refer to the USP for details on samplecollection and preparation for testing of nonsterile products.1

Procedure

For food samples, refer to appropriate standard references for details on test methods for performing MPN technique with enrichment using EE Broth Mossel Enrichment.6-7
For pharmaceutical samples, refer to USP General Chapter <62> for details on the examination of nonsterile products and tests for isolating Enterobacteriaceae using EE Broth Mossel Enrichment.1

Expected Results

Acid production causes the color of EE Broth Mossel Enrichment to become yellow. A negative reaction results in no color change and the medium remains green.

† QC testing performed according to USP/EP/JP performance specifications.

Free Shipping within the Continental USA

Celebrity Endorsements

1. United States Pharmacopeial Convention, Inc. 2008. The United States pharmacopeia 31/The national formulary 26, Supp. 1, 8-1-08, online. United States Pharmacopeial Convention, Inc., Rockville, Md.

2. European Directorate for the Quality of Medicines and Healthcare. 2008. The European pharmacopoeia, 6th ed., Supp. 1, 4-1-2008, online. European Directorate for the Quality of Medicines and Healthcare, Council of Europe, 226 Avenue de Colmar BP907-, F-67029 Strasbourg Cedex 1, France.

3. Japanese Ministry of Health, Labour and Welfare. 2006. The Japanese pharmacopoeia, 15th ed., online. Japanese Ministry of Health, Labour and Welfare.

4. Mossel, Vissar and Cornelisen. 1963. J. Appl. Bacteriol. 26:444.

5. Hartman and Minnich. 1981. J. Food Prot. 44:385.

6. International Organization for Standardization. 2004 Microbiology of food and animal feeding stuffs – horizontal methods for the detection and enumeration of Enterobacteriaceae – Part 1: Detection and enumeration by MPN technique with pre-enrichment. ISO 21528-1, 1st ed., 2004-08-15. International Organization for Standardization, Geneva, Switzerland.

7. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.

Add to Wish List

Click the button below to add the EE Broth Mossel Enrichment 500g to your wish list.

Let's Stay in Touch


Recently Viewed...


Terms of Service & Privacy Policy | Sitemap | P212121 Company Information

All prices are in USD Copyright 2017 P212121, LLC.