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Zeocin 1g

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  CAS Number:   11006-33-0
  Chemical Formula:   C55H85N20O21S2Cu · HCl
  Molecular Weight:   1526.5 g/mol


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10 mg/mL ZeocinTM Stock Solution Protocol

Making the stock solution:

  1. Weigh out 1 g of Zeocin.
  2. Ad 10 ml of sterile H2O, or Hepes Buffer (5 g/L, pH 7.25). Dissolve completely by mixing or vortexing.
  3. Prewet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water.
  4. Sterilize ZeocinTM Stock through the prepared 0.2 µm syringe filter.
  5. Store stock solution at -20°C. Solution will be stable for up to 1 year at his temperature.

Recommended selection concentrations: 

  • Bacterial cells (E. Coli, etc): 25-50 µg/mL
  • Mamamlian Cells (cell line dependent): 50-40µg/mL

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