TSTA3, Human

(FX, P35B, SDR4E1, GDP-L-fucose synthase, Protein FX.)

TSTA3 is a NADP(H)-binding protein. TSTA3 catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of numerous fucosyltransferases that take part in the expression of several glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in TSTA3 result in leukocyte adhesion deficiency, type II.

TSTA3 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 341 amino acids (1-321 a.a.) and having a molecular mass of 38 kDa. The TSTA3 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Purity: Greater than 90.0% as determined by SDS-PAGE.

Source: Escherichia Coli.

Physical Appearance: Sterile filtered colorless solution.

Formulation: 0.5mg/ml solution containing 20mM Tris pH-8, 2mM DTT, 0.0.5M NaCl & 10% glycerol.

TSTA3 General Information

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD TAQTRALFEK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHMNDNVLHS AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSN FGYSYAKRMI DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS SGSALTVWGT GNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV SIKEAAEAVV EAMDFHGEVT FDTTKSDGQF KKTASNSKLR TYLPDFRFTP FKQAVKETCA WFTDNYEQAR K.

Storage:
TSTA3 Human although stable at 4C for 1 week, should be stored desiccated below -18C. Please prevent freeze thaw cycles.

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