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BHMT, Human

Price:
$93.00
SKU:
PS-ENZ-292-10UG
Quantity:

BHMT, Human

(BHMT; Betaine Homocysteine S-Methyltransferase 1; BHMT1; EC 2.1.1.5)

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis.

Betaine Homocysteine S-Methyltransferase Human Recombinant fused to His Tag at N-terminal produced in E. coli is a single, non-glycosylated, polypeptide chain containing 443 amino acids (21-236) and having a molecular mass of 49.2 kDa. The BHMT is purified by proprietary chromatographic techniques.

Purity > 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Source: Escherichia Coli

Physical Appearance: Sterile Filtered clear colorless solution 1 mg/ml.

Formulation: The BHMT solution contains 20mM Tris-HCl pH-7.5 and 20% glycerol.

Reaction catalysed
Trimethylammonioacetate + L-homocysteine <=> dimethylglycine + L-methionine

BHMT General Information

Amino Acid Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFIDLPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPPASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ.

Storage:
BHMT Recombinant Human although stable at 4°C for 30 days, should be stored desiccated below -20°C for periods greater than 30 days. Please avoid freeze-thaw cycles.

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