Size | 100μg/vial |
Form | Liquid |
Ig type | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human HSP27, different from the related mouse sequence by two amino acids. |
Application | WB,IHC-P |
Contents | Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. |
Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
It can also be aliquotted and stored frozen at -20˚C for a longer time. | |
Avoid repeated freezing and thawing. | |
The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. The deduced 199-amino acid HSP27 protein shows sequence similarity to mammalian alpha-crystallins. Approximately 20% of its residues are susceptible to phosphorylation. The HSP27 gene, which is mapped to 7q11.23 and has 3 exons, produced a 2.2-kb transcript in an in vitro transcription assay. Decreasing ROS in cells expressing mutant huntingtin, HSP27 protects cells against oxidative stress. In other words, HSP27 is a suppressor of polyglutamine (polyQ)-mediated cell death. Furthermore, MAPKAPK5 is a major stress-activated kinase that can phosphorylate HSP27 in vitro. |
Concentration |
Tested Species |
Concluded Species |
Antigen Retrieval |
|
WB |
0.1-0.5μg/ml |
Hu |
Rat, Ms |
- |
IHC-P |
0.5-1μg/ml |
Hu |
Rat, Ms |
By Heat |