(Bacterial leucyl aminopeptidase, EC 3.4.11.10.)
The 31.4 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
Purity: Greater than 98.0% as determined by RP-HPLC and SDS-PAGE analyses.
Source: Aeromonas Proteolytica.
Physical Appearance: Sterile filtered liquid formulation.
Formulation: Buffered solution containing 10mM Tris-HCl, 100mM NaCl and 5µM ZnSO4, pH 8.0.
Storage:
Two years when stored at -20°C, 2 weeks at 4°C.
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