Alkaline Phosphatase Bovine

(EC 3.1.3.1, IAP, AP.)

The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

Purity: 95% pure by Gel Filtration.

Source: Calf Intestine.

Physical Appearance: Sterile Filtered clear colorless solution.

Formulation: 50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.

Alkaline Phosphatase Bovine General Information

Storage:
AP should be stored at 4°C. Please Do-Not freeze.

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