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ACAT1, Human

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$93.00
SKU:
PS-ENZ-665-5UG
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ACAT1, Human

(Acetyl-CoA acetyltransferase, mitochondrial; EC 2.3.1.9; Acetoacetyl-CoA thiolase; T2; ACAT1; ACAT; MAT; THIL; beta-acetoacetyl coenzyme A thiolase; 3-oxothiolase; acetyl coenzyme A thiolase; acetyl-CoA acetyltransferase; acetyl-CoA:N-acetyltransferase; thiolase II; CAS: 9027-46-7)

Acetoacetyl-CoA thiolase (ACAT1) is an enzyme member of the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. The ACAT1 enzyme catalyzes the reversible formation of acetoacetyl-CoA from 2 molecules of acetyl-CoA. ACAT1 plays a part in lipoprotein compilation and dietary cholesterol absorption. Added to its acyltransferase activity, ACAT1 acts as a ligase.

Catalytic Activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA

ACAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 417 amino acids (34-427) and having a molecular mass of 43.8 kDa. ACAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Purity: Greater than 95% as determined by SDS-PAGE.

Source: E.coli.

Physical Appearance: Sterile Filtered colorless solution.

Formulation: The ACAT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl, 10% glycerol and 1mM DTT.

ACAT1 General Information

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSVSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL

Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

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Celebrity Endorsements

Lynen and Ochoa. Enzymes of fatty acid metabolism. Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):299-314.

Stern, et. al. Enzymes of ketone body metabolism. I. Purification of an acetoacetate-synthesizing enzyme from ox liver. J Biol Chem. 1960 Feb;235:313-7.

Klein, et. al. Fatty acid degradation in Escherichia coli. European Journal of Biochemistry. Volume 19, Issue 3, pages 442–450, April 1971. DOI: 10.1111/j.1432-1033.1971.tb01334.x

Perry, et. al. Coenzyme A-acetylating enzymes in Alzheimer's disease: possible cholinergic 'compartment'of pyruvate dehydrogenase. Neuroscience Letters. Volume 18, Issue 1, 15 May 1980, Pages 105–110.

Fitch, et. al. Microbiological screening of mevalonate-suppressive minor plant constituents. J. Agric. Food Chem., 1989, 37 (3), pp 687–691
DOI: 10.1021/jf00087a024

Petersen and Bennett. Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (thiolase; EC 2.3. 1.9) gene. Appl. Environ. Microbiol. September 1991 vol. 57 no. 9 2735-2741.

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