Formula: per liter: Peptone 20g, Yeast Extract 10g, Glucose 20g.
Usage: dissolve 50g of mix in 1 liter of water, adjust PH to 6.8, autoclave at 121oC for 15 min.
Storage: in tightly-closed container at room teperature.
Peptone 20 g
Yeast Extract 10 g
Glucose 20 g
Dissolve 50 grams of mix in 1 liter of water
Adjust pH to 6.8
Autoclave at 121 °C for 15 min.
Tightly closed at room temperature.
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Hanko, V. P.; et. al. (2004) Determination of amino acids in cell culture and fermentation broth media using anion-exchange chromatography with integrated pulsed amperometric detection. J Biomol Tech. 15(4). 317–324.
-This paper works to determine the free amino acids in cell culture and fermentation broth media including YPD broth.