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Triton X 100 500ml

Price:
$19.50
SKU:
RP-111036-500ML
Quantity:

Triton X-100

(TX-100; octoxynol 9; 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether)

Scintillation Grade

  CAS Number:
  9002-93-1
  Chemical Formula:   C34H62O11
  Molecular Weight:   647 g/mol

 

Triton X-100 is a non-ionic detergent that is suitable for solubilizing membrane proteins.

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Product Specifications:

  Properties   Specifications   Result
  Appearance   Clear w/slight yellow   Pass
  Cloud Pt, 1% H20   +26.6° to +28.8°   +28.0°
  Color, Pt-Co   <60   9

 

Please contact for current lot details.


Protocol: Triton X-100 for the Recovery Inclusion Bodies

1) Centrifuge the cell lysate at maximum speed for 15 minutes at 4 degrees Celsius in a microcentrifuge.

2) Decant the supernatant.  Resuspend the pellet in 9 volumes of Cell lysis buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA (pH 8.0), 100 mM NaCl, 0.5 % Triton X-100) on ice.

3) Store the suspension for 5 minutes at room temperature.

4) Centrifuge the tube at maximum speed for 15 minutes at 4 degrees in a microfuge.

5) Decant the supernatant and set it aside for the next step.  Resuspend the pellet in 100 ul of H2O.

6)  Remove 10 ul samples of the supernatant and of the reuspended pellet.  Mix each sample with 10 ul of 2x SDS gel loading buffer and analyze the samples of SDS-PAGE to determine which fraction contains the protein of interest.

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Celebrity Endorsements

Marston, SB, Smith, CWJ. (1984) Purification and properties of Ca2+ - regulated thin filaments and F-actin from sheep aorta smooth muscle. Journal of Muscle Research and Cell Motility.

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