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Soytone Culture Media 500g

Price:
$78.00
SKU:
BD-243620-500G
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Soytone Culture Media

Intended Use

Soytone are used in media for the cultivation of a large variety of organisms, including fungi.

Summary and Explanation

Select Soytone demonstrates excellent growth support for Escherichia coli. Select Soytone is also used in molecular genetics media such as Select APS™ Super Broth. Subtle differences in the hydrolysation process give Select Soytone improved performance in cell culture.
Soytone was found to be effective in the recovery of stressed E. coli.2 It was found that Bacto Soytone with the addition of 7 vitamins replaced yeast extract as an economical alternative for the production of lactic acid by Lactobacillus rhamnosus.3 It should be noted that Bacto Soytone utilizes an enzyme in the digestion of the soy flour that has been sourced from animal.
Media formulations containing Phytone Peptone or Bacto Soytone are specified in various standard methods for multiple applications.4-8

Principles of the Procedure

Select Soytone and Bacto Soytone are enzymatic digests of soybean meal. The nitrogen source in the soy peptones contains naturally occurring high concentrations of vitamins and carbohydrates of soybean.

Identity Specifications
Select Soytone
Dehydrated Appearance: Tan, free-flowing, homogeneous powder.
Solution:                       2.0% solution, soluble in purified water. Solution is clear to moderately hazy.
Reaction of 2.0%
Solution at 25°C:           pH 6.5-7.5
Bacto Soytone
Dehydrated Appearance: Light to medium tan, free-flowing, homogeneous.
Solution:                       2.0% solution, soluble in purified water. Solution is light to medium amber,
                                   clear to very slightly hazy. A small amount of precipitate is acceptable.
Reaction of 2.0%
Solution at 25°C:           pH 6.5-7.5
Cultural Response
Biochemical Reactions
Bacto Soytone
Prepare sterile solutions of Bacto Soytone as directed below. Adjust final pH to 7.2-7.4. Inoculate and incubate at 35 ± 2°C for 18-48 hours.

TEST TEST SOLUTION ORGANISM ATCC™ INOCULUM CFU RESULT
Fermentable Carbohydrates 2% Escherichia coli 25922  ~107 Slight positive
Indole Production  0.1% Escherichia coli 29552  0.1 mL, undiluted  Positive
Acetylmethylcarbinol 
Production
 0.1% with 0.5% dextrose Enterobacter aerogenes 13048 0.1 mL, undiluted  Positive

Hydrogen Sulfide  
Production

1%  Salmonella enterica 14028  0.1 mL, undiluted  Positive

Growth Response
Bacto Soytone
Prepare a sterile solution with 2% Bacto Soytone, 0.5% sodium chloride and 1.5% agar. Adjust final pH to 7.2-7.4. Inoculate and incubate plates at 35 ± 2°C for 18-48 hours.

ORGANISM  ATCC™ INOCULUM CFU RECOVERY
Staphylococcus aureus 6538P 103-104 Good
Streptococcus pneumoniae 6305 103-104 Good
Streptococcus pyogenes 49117 104-105 Good

Typical Analysis

Refer to Product Tables in the Reference Guide section of this manual.

Directions for Preparation from Dehydrated Product

Refer to the final concentration of Select Soytone and Bacto Soytone in the formula of the medium being prepared. Add appropriate product as required.

Procedure

See appropriate references for specific procedures using Select Soytone and Bacto Soytone

Expected Results

Refer to appropriate references and procedures for results.

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Celebrity Endorsements

1. Power (ed.). 1988. Manual of BBL™ products and laboratory procedures, 6th ed. Becton Dickinson Microbiology Systems, Cockeysville, Md. 2. Chou and Cheng. 2000. Int. J. Food Microbiol. 61:127.
3. Kwon, Lee, Lee, Chang, Keun and Chang. 2000. Enzyme Microb. Technol. 26:209. 4. Horowitz (ed.). 2007. Official methods of analysis of AOAC International, 18th ed., online. AOAC International, Gaithersburg, Md. 5. U.S. Food and Drug Administration. 2001. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md. 6. U.S. Environmental Protection Agency. 2000. Improved enumeration methods for the recreational water quality indicators: Enterococci and Escherichia coli. EPA-821/R-97/004. Office of Water, USEPA, Washington, D.C. 7. Eaton, Rice and Baird (ed.). 2005. Standard methods for the examination of water and wastewater, 21st ed., online. American Public Health Association, Washington D.C. 8. U.S. Department of Agriculture. Microbiolo

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