Kanamycin is an aminoglycoside antibiotic and has a bactericidal action against many gram-negative bacteria. Kanamycin is used as a selective agent for the incorporation of the NPT II (APH3) gene in plant tissues.
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Kowalinski, E.; et. al. (2007) Expression, purification, crystallization and preliminary crystallographic analysis of the proliferation-associated protein Ebp1. Acta Crystallographica Section F. F6., 768-770.
- The paper describes their cell expression. The method and concentration of kanamycin used has become fairly standardized. In this case, cells were grown at 310 K in lysogeny broth medium complemented with 50 mg/ml kanamycin.
Klink, B.u.; Scheidig, A.J. (2010) New insight into the dynamic properties and the active site architecture of H-Ras p 21 revealed by X-ray crystallography at very high resolution. BMC Structural Biology. 10:38.
Cells were grown in 10 l Standard I-medium (50 μg/ml ampicillin, 50 μg/ml kanamycin) at 37°C.