Kanamycin Monosulfate

Molecular Biology Grade

Approximately 800 ug/mg
Kanamycin B: 0.82%

Kanamycin is an antibiotic typically used against prokaryotes. It is an aminoglycoside that indirectly interrupts protein synthesis in non-resistant cells by binding to the ribosome and disrupting translocation of tRNA during translation.

Aminoglycosides like kanamycin "irreversibly" bind to specific 30S-subunit proteins and 16S rRNA. Specifically, kanamycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes.

Store dessicated at -20°C
Soluble in water

Protocol Using Kanamycin:

How to make a 50 mg/ml Kanamycin Stock Solution

View this method on protocols.io and use it directly at your bench

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