dNTP Mix

HPLC grade 5X500ul/Kit, 10 mM each

Purity > 99%

10 mM dNTP mixture is suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, TdT tailing reactions, and dilution of radiolabeled dNTPs. These nucleotides are supplied as convenient, ready-to-use solution at a concentration of 10 mM in Tris-HCl (pH 7.5). The dNTP Mixture contains all four of the dNTPs, each at a final concentration of 10 mM.

Quality Control:

1. The concentration is verified by optical density spectrophotometry.
2. This preparation is free of DNase and RNase contamination as determined by incubation of the 4 dNTP's with radioactive substrates.
3. The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99 %.
4. This lot of dNTPs has been performance tested with Sequenase, Taq DNA polymerase, pfu DNA polymerase, and T7 DNA polymerase.

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