CHAPSO is a zwitterionic detergent often used to solublize proteins and for the solubilization of integral membrane proteins. CHAPSO differs from CHAPS in having an additional hydroxyl group between the ammonio and sulfonate moieties.
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Cladera, J.; et. al. (1997) Liposome solubilization and membrane protein reconstitution using Chaps and Chapso. European Journal of Biochemistry. Vol. 243. 798-804.
-The paper examines the solubilization of the liposome and reconstitution of two transport proteins using CHAPS and CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-l-propanesulfonate). The solubilization of unilamellar liposomes was followed by absorption experiments and the process was shown to fit well to the three-stage model described by other detergents. The solubilization parameters have been determined and the detergent to phospholipid ratios at which the lamellar-to-micellar transition initiates are described. The use of CHAPSO and CHAPS for membrane protein reconstitution studies yields a reproducible method of achieving active proteoliposomes, homogeneous in size with a low permeability and therefore is suited for transport measurements.