Loading... Please wait...
  • My Account
  • Order Status
  • Wish Lists

Cesium Chloride 100 g

Price:
$66.75
SKU:
RP-C68050-100.0
Quantity:

Cesium Chloride

Purity > 99.9 %

CAS Number:
  7647-17-8
Chemical Formula:   CsCl
Molecular Weight:   168.36 g/mol

 

Soluble in: Water

Store at: Room Temperature

Cesium chloride is used in the preparation of density gradients for isolation of plasmids from DNA, phage and/or cellular RNA.  Cesium chloride is also used in protein crystallization in order to create a heavy atom derivative.

Free Shipping within the Continental USA


Protocol for the Preparation of Bacterial DNA (Large Scale)

Materials

Cesium Chloride

CsCl with saturated isopanol or water saturated butanol

Beckman JA-20 and VTi80 rotors or similar

1) Grow 100 ml of bacterial culture until saturation is reached.  Centrifuge cells for 10 minutes at 4000 x g.

2) Resuspend the pellet in 9.5 ml TE buffer with 0.5 ml of 10 % SDS and 50 ul of 20 mg/ml proteinase K and incubate at 37 degrees Celcius for 1 hour.

3) Add 1.8 ml of 5 M NaCl and mix.  Add 1.5 ml CTAB/NaCl solution, mix and incubate 20 minutes at 65 degrees Celcius.

4) Add an equivalent volume of cholorform/isoamyl alcohol, extract and centrifuge 10 min at 600 x g at room temperature.  Transfer supernatant with a wide-bore pipet to fresh tube.

5) Add 0.6 vol isopropanol and mix gently until DNA precipitates.  Transfer precipitate with a sealed Pasteur pipet to 1 ml of 70 % ethanol and wash.  Centrifuge 5 minutes at 10,000 x g and discard supernatant.  Resuspend pellet in 4 ml TE buffer.

6) Determine the DNA concentration with a spectrophotometer and adjust to and adjust to 50  to 100 ug/ml.

7) Add 4.3 g CsCl into 4 ml TE buffer and it dissolves.  Add 200 ul of 10 mg/ml ethidium bromide.  Transfer to 4 ml centrifuge tubes, adjust volume and balance tubes with CsCl in TE buffer (1.05 g/ml).  Seal tubes and centrifuge 4 hour at 430,000 x g, 15 degrees Celcius or overnight at 250,000 x g.

8) Visualize gradient under longwave UV lamp.  Remove band with 15-G needle and 3 ml plastic syringe.

9) Remove ethidium bromide by sequential extractions with CsCl saturated with isopropanol or water saturated butanol.

10) Dialyze overnight against 2 L TE buffer to remove CsCl. If needed precipitate DNA and resuspend at desired concentration.


Comparable Items:

  Alfa Aesar    42832-14
  Alfa Aesar    42832-06
  Alfa Aesar    35689-18
  Alfa Aesar    10993-22
  Alfa Aesar    89188-09
  Alfa Aesar    89188-18
  Alfa Aesar    35690-14
  Alfa Aesar    42832-22
  Alfa Aesar    12886-30
  Alfa Aesar    35690-06
  Alfa Aesar    10993-14
  Alfa Aesar    10018-22
  Alfa Aesar    10018-14
  Alfa Aesar    87640-18
  Alfa Aesar    J65950-14
  Alfa Aesar    87640-09
  Alfa Aesar    35689-09
  Alfa Aesar    12886-18
  Alfa Aesar    43720-18
  Alfa Aesar    43720-30
  Alfa Aesar    43720-09
  Alfa Aesar    J65950-22
  Amresco    0415-500G
  Amresco    0415-250G
  Amresco    0415-100G
  Amresco    0415-50G
  EMD Millipore    1.02041.1000
  EMD Millipore    1.02038.0100
  EMD Millipore    1.02039.0250
  EMD Millipore    1.02039.0050
  EMD Millipore    1.02038.0025
  EMD Millipore    1.02039.1000
  Spectrum Chemicals    CE117-100GM
  Spectrum Chemicals    CE117-1KG
  Spectrum Chemicals    CE117-25GM
  Spectrum Chemicals    CE117-5KGBL
  Spectrum Chemicals    CE117-500GM
  VWR International   

Add to Wish List

Click the button below to add the Cesium Chloride 100 g to your wish list.

Let's Stay in Touch


Recently Viewed...


Terms of Service & Privacy Policy | Sitemap | P212121 Company Information

All prices are in USD Copyright 2017 P212121, LLC.