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Carbenicillin Disodium Salt 5 g

Price:
$46.00
SKU:
TWA-C-103-5
Quantity:

Carbenicillin Disodium Salt

Molecular Biology Grade

  CAS Number:
  4800-94-6
  Chemical Formula:
  Na2C17H16N2O6S
  Molecular Weight:
  422.4 g/mol

 

Carbenicillin an analog to ampicillin is an inhibitor of bacterial cell wall synthesis by disrupting peptidoglycan cross-linking.  Specifically, transpeptidases is inactivated within the cytosollic of the bacterial cell membrane.  High activity against gram-negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. B-lactamase sensitive.

Long Term Storage dessicated at -20°C
Soluble in water

Free Shipping within the Continental USA


Preparation of 50 mg/ml Carbenicillin Stock Solution

(Applicable for Salmonella typhimurium or E. coli)

  1. Take 50 mg of Carbenicillin and add 1 ml of molecular biology grade water to get a final concentration of 50 mg/ml.
  2. Use a vortex to dissolve.
  3. Draw solution into 1 ml syringe.
  4. Pass solution through a 0.22-micron filter into a fresh sterile container.
  5. Store at -20 °C
  6. When ready to use add 2 μl of stock solution per 1 ml of media to get a final concentration of 2 μl/ml.

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Celebrity Endorsements

Mancini, E. J.; et. al. (2007) Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Å resolution. Protein Science. 16(10). 2294–2300.

Expression in Escherichia coli Rosetta (DE3) pLysS in 1 L of Overnight Express instant TB medium (Novagen) containing 50 μg/mL carbenicillin, 35 μg/mL chloramphenicol, and 1% glucose.

Barglow, K. T.; et. al. (2008) Functional Proteomic and Structural Insights into Molecular Recognition in the Nitrilase Family Enzymes. Biochemistry. 47(51): 13514–13523.

-Cells were grown in LB media with 75 mg/L carbenicillin to an OD600 of 0.5 with shaking at 37 °C and protein expression induced with 1 mM IPTG.

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