Carbenicillin an analog to ampicillin is an inhibitor of bacterial cell wall synthesis by disrupting peptidoglycan cross-linking. Specifically, transpeptidases is inactivated within the cytosollic of the bacterial cell membrane. High activity against gram-negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. B-lactamase sensitive.
Long Term Storage dessicated at -20°C Soluble in water
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Preparation of 50 mg/ml Carbenicillin Stock Solution
(Applicable for Salmonella typhimurium or E. coli)
Take 50 mg of Carbenicillin and add 1 ml of molecular biology grade water to get a final concentration of 50 mg/ml.
Use a vortex to dissolve.
Draw solution into 1 ml syringe.
Pass solution through a 0.22-micron filter into a fresh sterile container.
Store at -20 °C
When ready to use add 2 μl of stock solution per 1 ml of media to get a final concentration of 2 μl/ml.
Mancini, E. J.; et. al. (2007) Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Å resolution. Protein Science. 16(10). 2294–2300.
Expression in Escherichia coli Rosetta (DE3) pLysS in 1 L of Overnight Express instant TB medium (Novagen) containing 50 μg/mL carbenicillin, 35 μg/mL chloramphenicol, and 1% glucose.
Barglow, K. T.; et. al. (2008) Functional Proteomic and Structural Insights into Molecular Recognition in the Nitrilase Family Enzymes. Biochemistry. 47(51): 13514–13523.
-Cells were grown in LB media with 75 mg/L carbenicillin to an OD600 of 0.5 with shaking at 37 °C and protein expression induced with 1 mM IPTG.