(5-Bromo-3-indoxyl-β-D-galactopyranoside; Blue-Gal; Lapis™-β-D-Gal)
Purity > 99.0 %
|Molecular Weight:||374.2 g/mol|
Bluo-gal is used as an alternative to X-gal that produces a darker blue color, which is ideal for white/blue assay screening. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing bluo-gal.
Soluble in dimethylformamide (DMFA) and dimethyl sulfoxide (DMSO)
Store at -15°C, protect from light.
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