Bluo-Gal

(5-Bromo-3-indoxyl-β-D-galactopyranoside; Blue-Gal; Lapis™-β-D-Gal)

Purity > 99.0 %

Bluo-gal is used as an alternative to X-gal that produces a darker blue color, which is ideal for white/blue assay screening. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing bluo-gal.

Soluble in dimethylformamide (DMFA) and dimethyl sulfoxide (DMSO)

Store at -15°C, protect from light.

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