Biosate Peptone

Intended Use

Biosate Peptone is used as a component in microbiological culture media or in fermentation applications.

Summary and Explanation

Biosate Peptone is a mixed hydrolysate comprised of casein and yeast extract at a ratio of 65:35. The synergistic effect of two or more types of hydrolysates is well documented and has
been utilized for decades in culture media formulation. The combination of pancreatic digest of casein and yeast extract provides nutritional benefits that are not provided by the
components alone. It has been reported that the combined use of these two peptones has shown improved toxin production in clostridia.1,2 Additionally, the combination of pancreatic
digest of casein and yeast extract has been used successfully as components in media which supported the hatching and culture of Giardia spp. from cysts and the first-time culturing of
a nematode without the need of its symbiotic bacteria.3,4

Principles of the Procedure

Biosate Peptone provides nitrogen, amino acids and vitamins in microbiological culture media. In addition, the yeast extract component of the product provides proteins, carbohydrates
and some micronutrients.

Typical Analysis

Refer to Product Tables in the Reference Guide section of this manual.

Precautions5

1. Biosafety Level 2 practices, containment equipment and facilities are recommended for activities with clinical specimens of human or animal origin containing or potentially containing pathogenic Brucella spp.
2. Biosafety Level 3 practices, containment equipment and facilities are recommended for all manipulations of cultures of the pathogenic Brucella spp. and for experimental animal studies.

User Quality Control

Identity Specifications
Biosate Peptone
Dehydrated Appearance: Yellow-tan powder, fine, homogeneous, free of
                                    extraneous material.
Solution:                       2.0% solution, soluble in purified water. Solution
                                   is light to medium, yellow to tan, clear to slightly hazy.
Reaction of 2.0%
Solution at 25°C:           pH 6.3-7.5
Cultural Response
Biosate Peptone
Prepare a sterile solution of peptone agar using 10 g of Biosate Peptone, 2.5 g of sodium chloride and 6.5 g of agar in 500 mL of purified water. Adjust final pH to 7.2-7.4. Inoculate and incubate plates at 35 ± 2°C for 2-3 days (incubate Brucella abortus and Streptococcus pyogenes with 3-5% CO2).

*If this strain is not available, verify performance with a known isolate.

Directions for Preparation from Dehydrated Product

Refer to the final concentration of Biosate Peptone in the formula of the medium being prepared. Add product as required.

Procedure

See appropriate references for specific procedures using Biosate Peptone.

Expected Results

Refer to appropriate references and procedures for results.

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