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Agarose 100 g

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$75.00
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Agarose

Agarose LE is used in electrophoresis, chromatography, molecular biology and biochemistry techniques.  The most common application of our Agarose LE is for the separations of nucleic acids.  Agarose is a natural product that forms an inert matrix that is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies.  Being non-toxicity makes working with agarose easy and convenient.

Agarose LE (Low Electroendosmosis) is the highest quality with Agarose LE is between 0.2 – 23kbp depending on the concentration of Agarose LE.

Applications:

• High electrophoresis mobility
• Nucleic acid analytical and preparative electrophoresis
• Blotting assays
• Protein electrophoresis such as radial immunodiffusion

Features:

• Extraordinary mechanical resistance for more reliable and easier handling
• Excellent transparency of the gel and high visibility
• Exceptionally low absorption of staining agents
• Absence of toxicity (polyacrylamide is neurotoxic)
• Possibility of varying pore size in accordance with particle size by modifying the gel concentration
• Easy preparation of the gel by simple dilution in aqueous buffers either by standard boiling or microwaving
• Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures)

Storage:

Store in a dry place at 15-25°C

Technical Specifications:

CAS: 9012-36-6
EEO (Electroendosmosis) ≤ 0.12 Sulfate ≤ 0.1%
Gel Strength 1% ≥ 1200 g/cm2
Gelling temperature 36 ± 1.5 °C
Melthing tempterature 88 ± 1.5 °C
DNAse and RNAse activity not detected
DNA resolution ≥ 1000 bp finely resolved
Gel Background and DNA Binding very low

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Celebrity Endorsements

Thiessen, K. J. (1994) The use of two novel methods to grow protein crystals by microdialysis and vapor diffusion in an agarose gel. Acta Crystallographica Section D: Biological Crystallography. Vol 50. 491-495.

Lorber, B., et. al. (1999) Crystallization within agarose gel in microgravity improves the quality of thaumatin crystals. Acta Crystallographica Section D: Biological Crystallography. Vol 55. 1491-1494.

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