(DTT; (D,L)-1,4-Dithiothreitol; DL-Dithiothreitol; Cleland's Reagent; Cleland Reagent; threo-2,3-dihydroxy-1,4-dithiolbutane)
Purity > 99.5 %
Molecular Biology Grade
|Molecular Weight:||154.25 g/mol|
Dithiothreitol is a commonly used reducing agent and is soluble in a range of solvents including water, ethanol, acetone and ethyl acetate. DTT is quite hygroscpoic and in some applications has been replaced by TCEP HCl. The use of dithiothreitol (DTT) was popularized by William Wallace Cleland who first published on its reducing potential in 1964. This led to dithiothreitol being referred to as Cleland's regeant. DTT has a reducing potential of -0.33 V at pH 7. DTT should only be used as a reducing agent above a pH 7 since the thiol group needs to deprotonated becoming a thiolate (S-) species. The thiolate group is responsible for breaking apart the disulfide bonds within proteins.
DTT is listed under two CAS numbers 27565-41-9 and 3483-12-3.
Store: -20 °C
Free within the Continental USA
Manufacture and Expiration Data available upon request.
|Appearance||White crystalline powder||Corresponds|
|Absorbance (280 nm)||Colorless, clear||0.1 Molar|
|% Oxidized DTT||0.3 %||Conforms|
|Melting Point||40-44 °C||42 °C|
|pH 0.1 M||N/A||4-6|